Screening and Identification of Polyhydroxybutyrate (PHB) producing bacteria obtained from Incinerator Dump sites

Durga Devi et al.,

Bacillus | PHB

Vol: 11; Iss. 8; Aug 25 | ISSN: 2454-5422

 We are studying microbial strains in ecosystems that can manufacture bioplastics with high efficiency and environmental sustainability. Some bacteria and archaea, such as Bacillus, Azotobacter, Rhizobium, and Pseudomonas, among others, produce polyhydroxybutyrate (PHB), a biodegradable polymer. PHB is a conceivable substitute for synthetic polymer due to their rapid decomposition in the natural environment. The purpose of this study is to screen and identify PHB-producing bacteria from various soil sources. For this purpose, the soil sample was taken from the main incinerator dump sites in Puliyankulam, Madurai district. The PHB production was tested by the bacterial isolates and was performed through a staining method using Sudan Black B. The PHB-producing strains appeared in bluish colonies selected for biochemical and molecular studies. The selected PHB strains are used for the optimisation process with different parameters such as pH, temperature, carbon, nitrogen, etc. The motive of this study is to produce PHB-producing bacteria from incinerator dump sites. The health concerns of this study are beneficial for both ecologically friendly and cost-effective. Because the petrochemical plastics have negative impacts on human health and the environment. This study is more useful for the environment, and it reduces the level of environmental pollution.

Durga Devi A, PG Student, PG & Research Department of Microbiology, V.H.N.Senthikumara Nadar College (Autonomous), Virudhunagar, Tamilnadu, India