Recovery of AutoFluorescent Pigment from Different Species of Marine Micro Algae

Sathiya et al.,

Micro algae | Chlorophylls

Vol: 11; Iss. 7; Jul 25 | ISSN: 2454-5422

In the present work, two different species of microalgae: Spirogyra and Volvox were collected from the Palk Bay region and were selected as raw materials for the recovery of multifunctional extracts. After sampling, the micro algal samples are viewed under the fluorescent microscope for biofluorescent emission. The presence of pigments which absorb light energy and them reemit it at a longer wavelength, creating a visible fluorescent glow. To Prepare an appropriate culture medium like f/2 medium (Guillards Medium) to promoting micro algal growth at 18- 25ºC for several days to weeks under 12:12hours light/dark conditions and50–100 µmol photons m⁻² s⁻¹.Ultrasonic assisted extraction (UAE) is a process that uses sound waves to break down particles in the algal culture using different solvents was applied under specific optimized conditions. Organic solvents such as hexane, acetone and ethanol, as well as food grade solvents, such as, soybean oil and phosphate buffer (pH=7) were used. 10-20 mL of extraction solvent are sonicated at 40 kHz for 10 mins. The total carotenoid and chlorophyll a content in the extracts was determined for all species using UV-Visspectrophotometry. (Rodrigo Martins et al 2023). Taking into consideration the nature of the solvent used and the total pigment content, acetone and ethanol can be replaced by limonene for the extraction of total chlorophyll from Spirogyra and Volvox. HPLC (High Performance Liquid Chromatography) pigment analysis for micro algae to identify and quantify the various photosynthetic pigments present in microalgae, such as chlorophyll, Xanthophyll, carotenoids, and phycobiliproteins by separating them with a diode array detector (DAD).(Guillermo Linares and Meliza Lindsay Rojas et al 2022).

Dr. K. Jayakumar, Associate Professor & Head, Department of Animal Behaviour & Physiology, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamilnadu, India.