Assessment of biofilm formation and antibiofilm agent efficacy against Uropathogens: antibiotic susceptibility and molecular identification

Priyavadhana et al.,

Antibiotic | Biofilm

Vol: 11; Iss. 7; Jul 25 | ISSN: 2454-5422

The formation of biofilms by uropathogens is a major concern in urinary tract infections (UTIs), contributing to antibiotic resistance and chronic infections. This study aimed to assess the biofilm-forming ability of uropathogens and evaluate the efficacy of antibiofilm agents in combating these infections. We isolated and identifying uropathogens from urine samples using standard microbiological methods and confirmed by bacterial identification through molecular techniques. The ability of the selected isolates to produce biofilms was primarily confirmed using crystal violet assay. Antibiotic susceptibility profiles were determined by the disc diffusion method. Antibiofilm agents were tested for them to reduce biofilm formation.  Our results may be indicated that significant variability in biofilm formation among uropathogens, in contrast some of the strains exhibited strong biofilm production. This study emphasizes the need for alternative strategies in the management of biofilm-associated UTIs, antibiotic susceptibility testing and   molecular identification, and the development of effective antibiofilm agents to combat these persistent urinary infections.

Dr. C. Edward Raja, Assistant Professor, PG & Research Department of Microbiology, V.H.N.Senthikumara Nadar College (Autonomous), Virudhunagar, Tamilnadu, India.