Screening of Rutin and Isolation, Identification of Foodborne Drug Resistance Biofilm Producing Staphylococcus aureus and Escherichia coli from Fast Food

Karuppasamy et al.,

Rutin | Fast food

Vol: 11; Iss. 5; May. 25 | ISSN: 2454-5422

This study focused on the health concerns that arise foodborne drug resistance Biofilm producing Staphylococcus aureus and Escherichia coli isolated from several fast food. Public health and the food business worldwide are grappling with the establishment and spread of multi-drug resistance among these dangerous diseases. Moreover, the development of biofilms by foodborne pathogens has gathered significant interest in recent times because of the possible hazards associated with it, such as heightened susceptibility to antibiotics and the generation of virulence factors that result in food illness. This research explores the antibacterial activity of rutin, a flavonoid compound, isolated from banana leaves (Musa spp.) and its ability to suppress biofilm development by S.aureus and E.coli in fast foods. Bacteria were isolated from chicken fried rice and chicken burger samples homogenized and centrifuged at 4500 rpm for 15 minutes with culture techniques and characterized using biochemical tests, in which S. aureus and E. coli were the major isolates. Rutin was isolated from banana leaves with ethanol, and its phytochemical content was verified by ferric chloride test for the presence of phenolic compounds. The rutin extract was then analyzed using HPLC.

Muthulakshmi K, Assistant Professor, PG & Research Department of Microbiology, V.H.N.Senthikumara Nadar College (Autonomous), Virudhunagar, Tamilnadu