Isolation and identification of targeted uropathogenic bacteria from urinary tract infections

Chandru et al.,

Uropathogen | UTI etiology

Vol: 11; Iss. 10; Oct 25 | ISSN: 2454-5422

Urinary Tract Infections (UTIs) are common bacterial infections that affect the urinary system, primarily caused by pathogenic microorganisms. The objective of this study was to isolate and identify the microorganisms responsible for UTIs from urine samples obtained from patients exhibiting symptoms of infection. Urine samples were collected midstream to minimize contamination, and bacterial isolation was performed using standard microbiological techniques. Samples were cultured on selective and differential media, including Nutrient agar, Salmonella Shigella Agar (SS), Eosin Methylene Blue Agar (EMB), Mannitol salt agar, MacConkey agar and King’s B medium followed by incubation at 37°C for 12-24 hours. The bacterial colonies were then characterized based on their macroscopic appearance, Gram-staining properties, and biochemical tests, including catalase tests. Results indicated that the most common uropathogens were Escherichia coli, Klebsiella sp., Pseudomonas sp., Salmonella sp., and Staphylococcus sp. These findings contribute to a better understanding of the microbial etiology of UTIs.

Dr. Palpperumal S, Head & Assistant Professor, PG & Research Department of Microbiology, V.H.N. Senthikumara Nadar College (Autonomous), Virudhunagar, Tamilnadu