Soil Microbiomes in Suthamalli Reservoir, Tirunelveli District, Tamil Nadu, Using 16S rRNA Gene Sequencing

Mathar Fathima & Kannan

Microbiology | Metagenomic

Vol: 10; Iss:8 Aug 24 | ISSN: 2454-5422

Soil microbial diversity encompasses the variety of microorganisms found within the soil, including bacteria, fungi, archaea, and viruses.
Bacteria are among the many different types of microorganisms found in soil. In Suthamalli Reservoir, Tirunelveli District, Tamil Nadu, India,
soil samples were used in this study to investigate the variety of bacteria. DNA from the environment was extracted. The diversity indices have
been calculated and the Illumina MiSeq sequencing was conducted. Illumina MiSeq sequencing revealed 24 Phyla, five of which were abundant:
Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes and Bacillus. The investigated samples' soil microbial communities had a stable
community structure whereas becoming phylogenetically varied. 56 classes are represented with relative abundances of Gamma Proteobacteria,
Alpha proteobacteria, Beta proteobacteria, Actinobacteria, Chitinophgia, and Bacilli. Totally 253 genera were listed in the suthamalli soil sample
among these Proteus, Hydrogenophage, Gemmatimonas, Sphingomonas, Pseudomonas, Magnetospirillum, Flavobacterium, Serratia,
Prevotella, Lactobacillus, Enterococcus, Streptococcus, and Geobacter were most dominant in the study area.

M. Mathar Fathima, Research Scholar, Department of Environmental Studies, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai, Tamil Nadu, India