Production of Protease and Lipase Enzyme Producing Microorganism from Tannery Effluent

Production of Protease and Lipase Enzyme Producing Microorganism from Tannery Effluent

Somaprabha | Durairaj 

Tannery waste | Bacillus subtilis

Vol: 7; Iss:6; Jun 21 | ISSN: 2454-5422

Enzymatic dehairing in tanneries has been envisaged as an alternative to sulfides. Bacillus sp is industrially important enzymes producing a wide variety of extracellular enzymes (Protease, amylase, cellulose, lipases, pectinases and xylanases) producing microorganisms that are active and stable at high pH values. Bacillus sp isolated from local leather samples were collected from Salem. The present investigation on tannery effluent waste sample, was performed the collected tannery industry wastewater was serially diluted, spread plated, and characterized morphologically and biochemically. Screening of the isolates to detect the ability for extracellular protease and lipase enzyme by conforming measure of zone producing microorganism by Bacillus subtilis was studied. In order to enhance the lipase and protease enzyme supplementation of tween20/tween80 was performed. Determinations of protein contain for protease and lipase was carried out. In order to illustrated protease and lipase activity enzyme assay was performed. In order to enhance protease and lipase production tannery waste isolates. Optimization of experiments with pH, temperature, Incubation period, nitrogen source, carbon source, effect of NaCl was demonstrated. The optimum pH was found to be 7.0, Optimum temperature was found to be 37 °C. Among the carbon and nitrogen sources were tested glucose and urea was found to be best source for enhanced enzyme activity. Another important study was the incubation period for maximum production of enzymes as protease and lipase found at 24-48 hours. The agarose gel electrophoresis was run in order to check the plasmid DNA. The study was further extended to inoculate the lipase activity supplemented with different oils with carbon source was demonstrated. Besides dehairing activity of the crude protease enzyme were analyzed.