Isolation of pesticide degrading microorganism from water agriculture drainage

Reda Mohamed Taha et al.,

Pseudomonas | Diazinon 

Vol: 7; Iss:3; Mar. 21 | ISSN: 2454-5422

Diazinon is an organophosphorus often used in agriculture as a soil and foliar insecticide. Diazinon could be hazardous as a result of runoff from areas of application into nearby drains or ditches, that typically transport water to streams and lakes. In the present study, bacteria that has a high ability of diazinon degradation was isolated from agricultural drainage ditches (Fayoum, Egypt) by enrichment technique. Based on morphological, biochemical and 16S rDNA gene sequencing, a high isolated bacteria was recognized as Pseudomonas aeruginosa. Minimal medium supplemented with diazinon as sole carbon source used for the growth of a pure culture of P. aeruginosa. The effect of diazinon concentrations, temperature and pH on the bacterial growth as well as rate of diazinon degradation were studied. It was found that the maximum capability of diazinon degradation (73.46 %) was done at concentration 250 ppm of diazinon at pH value 7.0 and temperature 30 °C within 14 days. Therefore, P. aeruginosa could be used efficiently for cleaning up of pesticides in the environment specially contaminated water agricultural usage.