Effect of UV-B irradiation in in-vitro propagation of Vigna mungo cultivars

Periyakaruppiah et al.,                 

Ultraviolet-B | Vigna mungo               

Vol: 2; Iss: 5; May 16 | ISSN: 2454-5422

Callus induction was tried with leaf explants harvested from control and UV-B irradiated six cultivars of Vigna mungo (Vamban 1, Vamban 2, Vamban 3, Vamban 4, Vamban 5 and Vamban 6) to study their callus fresh weight and dry weight. Callus induction was recorded in Vamban 1, Vamban 2, Vamban 3, Vamban 4, Vamban 5 and Vamban 6 both from control and UV-B leaf explants harvested. UV-B delayed callus induction and depressed biomass accumulation. However, the callus fresh weight and dry weight were 100% compare to UV-B treated in all the cultivars of Vigna mungo under the control conditions. The cultivar Vamban 2 (V2) is the more sensitive one to the enhanced UV-B radiation treatment. The fresh weight and dry weight decreased by under UV-B treated plants 14%, 14%, 14%, 14%, 16% and 17% in the cultivar V1,V3, V4, V5, V6 and V2 respectively.