Exploring Molecular Mechanisms of ‘GONODOTROPIN RELEASING HORMONE-1’ (GNRH-1) in an Animal Model (Catla catla)
Exploring Molecular Mechanisms of ‘GONODOTROPIN RELEASING HORMONE-1’ (GNRH-1) in an Animal Model (CATLA CATLA).
Priyadharshini | Damodaran
Catla catla | Gonopro-FH
Vol: 2; Iss: 12; Dec 16 | ISSN: 2454-5422
Hormone-induced breeding of fish has been effectively achieved with Gonopro-FH, a salmon GnRH-1 analog, in several teleost fish but not quite successfully in the Catla catla. In order to analyze the rationale behind GnRH-1 ineffectiveness in this species, we investigated the effect of Gonopro-FH injection on the GnRH receptors of the ovary and other extra-pituitary organs, since GnRH receptors are known to be over-expressed during spawning. RT-PCR analysis revealed that the GnRH receptor-1 is present not only in the ovary, but also in the liver, brain and heart tissues of the Catla catla. However, the expression of the receptor declined in a time-dependent manner in response to Gonopro-FH injection in all the above tissues. Concomitant changes were observed in the gene expression of the ovary, liver, brain and heart tissues.