Lalitha Lakshmi et al.,    

carcinoma | T-Lymphocyte  

Volume: 5; Issue: 6; Jun-2019 | ISSN: 2454-5422

Lung carcinoma, a malignant condition characterized by uncontrolled cell growth in the tissues of lung. It is of two types viz, small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC). The later is the predominant carcinoma of lung constituting about 80% of lung cancer. Hence, advancements in identification of genetic variants of the immune genes (CTLA-4) that regulate the activation and proliferation of T lymphocytes examined for its susceptibility to lung cancer especially to NSCLC. The gene encoding Cytotoxic T-Lymphocyte antigen-4 (CTLA-4) is a promising candidate in the pathogenesis of NSCLC especially the polymorphism at position +49A/G (located in exon 1 leads to alanine to threonine substitution in leader sequence) of CTLA-4 gene in NSCLC. The objective of the present study is to investigate the prevalence of the +49A/G polymorphism in CTLA-4 gene and to assess the association of polymorphisms to the risk of lung cancer in and around Madurai (south Indian population). Blood samples obtained from Asirvatham specialty clinic, Madurai. Basic details like age, and smoking habit collected using a consent form. Genomic DNA isolated from blood was confirmed on agarose gel and subjected to PCR based RFLP analysis. Genotype classified by means of allele counting method. The results of the present study showed that the variant genotype of +49A/G polymorphism was lower in controls and patients. The distribution of genotypes was not in accordance to Hardy Weinberg equilibrium and the odds ratio revealed that there is 1.25 fold risk of developing cancer due to variant genotype of +49A/G SNP and  there is an association of polymorphism with NSCLC.