Optimization of hormones for the induction of callus, shoot and root buds of cotton, Gossypium hirsutum L. (Malvales: Malvaceae)

Edward et al.,

Hormones | Callus

Vol: 4; Iss: 1; Jan 18 | ISSN: 2454-5422

The present work is based on developing a protocol for the callus induction, shoot and root bud multiplication in cotton, Gossypium hirsutum L. from the shoot explant. The sterilized explant was inoculated in MS-medium containing various combination of auxin such as naphthalene acetic acid (NAA) and cytokinins such as kinetin and 6 penzyl amino purine (BAP). High frequency of callus induction was found in MS media with the combination BAP (0.5 mg/l) and NAA (1.5 mg/l) which was followed by BAP (0.5 mg/l) and IAA (1.5 mg/l). The highest frequency of shoot bud regeneration (57.2%) was observed on MS basal medium containing KIN (0.5 mg/l) and IAA (2.0 mg/l). The highest mean number of shoot bud multiplication was observed on MS medium with KIN (3.0 mg/l) and IAA (0.5 mg/l). The highest mean number of shoot bud multiplication was observed on MS medium with KIN (3.0 mg/l) and IAA (0.5 mg/l). The highest frequency of rooting was observed on MS medium containing 0.3 mg/l of IBA and 0.05 mg/l KIN. The protocol developed here can be used for genetic transformation experiments of Indian Cotton cultivars.